School of Life Sciences, The Chinese University of Hong Kong

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Professor Wong, Kam Bo

Address(Office): Rm 289, Science Centre South Block
(Lab): Rm 294, Science Centre South Block
Phone(Office): (852) 3943 8024
(Lab): (852) 3943 8073
Fax(852) 2603 7246
Emailkbwong@cuhk.edu.hk
Web

http://kbwongcuhk.wordpress.com

Education

1997 Ph. D. University of Cambridge
1993 M. Phi. The Chinese University of Hong Kong
1990 B. Sc. The Chinese University of Hong Kong

Positions

  • Professor, School of Life Sciences
  • Assembly of Fellow, S.H. Ho College

Research Interests

  • Using structural biology (NMR, X-ray crystallography and cryo-EM) to understand the molecular mechanism of life
  • The maturation pathway of urease
  • Vacuolar sorting in plant cells
  • Protein Engineering, Design, Simulation and Modelling

Representative Publications

  1. Yap Shing Nim, Ivan Yu Hang Fong, Justin Deme, Ka Lung Tsang, Joseph Caesar, steven Johnson, Longson Tsz Hin Pang, Nicholas Man Hon Yuen, Tin Long Chris Ng, Tung Choi, Yakie Yat Hei Wong, Susan M. Lea*, Kam-Bo Wong*. (2023) Science Advances, 9(16):eadf7790.
  2. Lei Yang, Ka-Ming Lee, Conny Wing-Heng Yu, Hirotatsu Imai, Andrew Kwok-Ho Choi, David K Banfield, Kosuke Ito, Toshio Uchiumi, Kam-Bo Wong*. (2022) Nucleic Acids Res, 50:5335-5348.
  3. Ka Lung Tsang, Kam-Bo Wong*. (2022) . Metallomics, 15(5):mfac003
  4. Hsi-En Tsao, Shu Nga Lui, Anthony Hiu-Fung Lo, Shuai Chen, Hiu Yan Wong, Chi-Kin Wong, Liwen Jiang, and Kam-Bo Wong*. (2022) Proc Natl Acad Sci USA, 119:e2111281119.
  5. Shuangli Sun, Lanlan Feng, Kin Pan Chung, Ka-Ming Lee, Hayley Hei-Yin Cheung, Mengqian Luo, Kaike Ren, Kai Ching Law, Liwen Jiang, Kam-Bo Wong, Xiaohong Zhuang. (2021) . Autophagy, 18(6):1350-1366.
  6. Kwok-Ho Andrew Choi, Lei Yang, Ka-Ming Lee, Conny Wing-Heng Yu, David K Banfield, Kosuke Ito, Toshio Uchiumi, Kam-Bo Wong*. (2019) . Biochemistry, 58:3744-3754.
  7. Yuen MH, Fong YH, Nim YS, Lau PH, Wong KB*. (2017) Proc Natl Acad Sci USA, 114:E10890–E10898
  8. Cheung MY, Li X, Miao R, Fong YH, Li KP, Yung YL, Yu MH, Wong KB*, Chen Z*, Lam HM*. (2016) Proc Natl Acad Sci USA. 113:2648-53
  9. Luo F, Fong YH, Zeng Y, Shen J, Jiang L, Wong KB*. (2014) Plant Cell, 26:3693-708
  10. Fong YH, Wong HC, Yeun MH, Lau PH, Chen YW, Wong KB*. (2013) PLoS Biol, 11:e1001678.
  11. Lee KM, Yusa K, Chu LO, Yu, CWH, Oono M, Miyoshi T, Ito K, Shaw PC, Wong KB*, Uchiumi T*. (2013) Nucleic Acids Res, 41:8776-8787
  12. Chuck CP, Chen C, Ke Z, Wan DC, Chow HF, Wong KB*. (2013) Eur J Med Chem, 59:1-6.
  13. Lee, K.M., Yu, W.H., Chiu, Y.H., Sze, K.H., Shaw, P.C., Wong, K.B*. (2012) , Nucleic Acids Res, 40:3172-82.
  14. Fong, Y.H., Wong, H.C., Chuck, C.P., Chen, Y.W., Sun, H., Wong, K.B.* (2011) , J Biol Chem 286:43241 (Selected as paper of the week).
  15. Chan C.H., Yu, T.H., Wong, K.B.* (2011) . PLoS One 6:e21624.
  16. Lam SY, Yeung RCY, Yu TH, Sze KH, Wong KB* (2011) PLoS Biol 9:e1001027
  17. Lee KM, Yu CW, Chan DS, Chiu TY, Zhu G, Sze KH, Shaw PC, Wong KB.* (2010) Nucleic Acids Res, 38:5206-5216.
  18. Too PH, Ma MK, Mak AN, Wong YT, Tung CK, Zhu G, Au SW, Wong KB*, Shaw PC*. (2009) Nucleic Acids Res, 37:602-610.
  19. Chan DS, Chu LO, Lee KM, Too PH, Ma KW, Sze KH, Zhu G, Shaw PC, Wong KB* (2007) Nucleic Acids Res, 35:1660-1672.
  20. Lee CF, Makhatadze GI, Wong KB* (2005) Biochemistry, 44:16817-16825.
  21. Lee CF, Allen MD, Bycroft M, Wong KB* (2005) J Mol Biol, 348:419-431.
  22. Cheung YY, Lam SY, Chu WK, Allen MD, Bycroft M, Wong KB* (2005) Biochemistry, 44:4601-4611.
  23. Wong KB*, Lee CF, Chan SH, Leung TY, Chen YW, Bycroft M (2003) Protein Sci, 12:1483-1495.
  24. Chen YW, Bycroft M, Wong KB* (2003) Biochemistry, 42:2857-2865.
 

Research Grants

2024-2026, GRF, Structural basis of cross-talking between the hydrogenase and urease maturation pathway, $1,595,040

2023-2025, GRF, How the receptor-homology-transmembrane-RING-H2 protein (RMR) recognizes and sorts its specific cargo to the vacuoles?, $1,180,218

2021-2024, GRF, Structural basis of specific interactions among metallochaperones that facilitate the nickel delivery in the urease maturation pathway, $1,175,732

2019-2022, CRF, A state-of-the-art X-ray diffraction facility for structural biology research in Hong Kong, $2,306,960

2018-2020, GRF, How SH3P2 interacts with ATG8 in the autophagosome biogenesis in Arabidopsis, $1,274,389

2017-2019, GRF, How the C-terminal vacuolar sorting detainment (ctVSD) of plant storage proteins is recognised by sorting receptors, $1,124,086

2016-2018, GRF, Structure-function study of eukaryotic proteins eL12 and P0 and their roles in protein translation, $974,892

2015-2017, GRF, Structure-function studies of urease accessory protein UreG – elucidating the conformational changes that couple GTP hydrolysis to urease maturation, $634,168

2014-2016, HMRF, Structure-based discovery of inhibitors of Helicobacter pylori urease maturation, $994,522

2013-2015, GRF, How vacuolar sorting receptor (VSR) proteins recognize their cargo-proteins – structure-function study of the ligand-binding domain of Arabidopsis VSR1 protein, $1,168,250

2012-2014, GRF, Structure-function studies of Helicobacter pylori urease accessory proteins UreF and UreH – protein engineering studies and structure determination of the ternary UreG/UreF/UreH complex, $1,050,000

2010-2012, GRF, Structure-function study of P-complex in eukaryotic ribosomal stalk – structure determination of the dimerization domain of P1/P2 complex by NMR spectroscopy, $1,333,220

2010-2011, RFCID, Profiling of substrate-specificity and rational design of broad-spectrum peptidomimetic inhibitors for main proteases of coronaviruses, $799,092

2008-2011, UGC Special Equipment Grant, Acquiring a high-field NMR spectrometer for chemical and structural biology – from macromolecular structures and dynamics to biomolecular interactions, $10,000,000

2008-2009, RFCID, Substrate specificity and rational design of peptidomimetic inhibitors for SARS coronavirus main protease, $775,008

2008-2010, GRF, Does Charge-charge interaction contribute more to protein stability at high temperatures? $639,261

2007-2009, GRF, Structure-function of hydrogenase maturation factor B (HypB) – structure determination of HypB by X-ray crystallography. $654,500

2006-2009, GRF, Is the reduced catalytic efficiency of a thermophilic acylphophsphatase a necessary consequence of enhanced stability and rigidity? $1,290,183

2003-2006, GRF, Structure-function study of ribosomal protein P2 – structure determination of human P2 by NMR spectroscopy, $1,491,000

2002-2005, GRF, Structural basis of thermostability of proteins – protein engineering of a thermophilic protein from a hyperthermophilic archaea Thermococcus celer, $1,394,000

2000-2003, GRF, Structure-function of a novel RNA binding motif – structure determination of a ribosomal protein L30e from Thermococcus celer by multi-dimensional NMR spectroscopy, $1,015,200

 

Professional Activities

Editoral Board, The Journal of MicrobiologyEditoral Board, Protein Engineering, Design and Selection